The rhizobiome of herbaceous plants in Clovelly and Sterkspruit soils of the Stevenson–Hamilton supersite

Koedoe - African Protected Area Conservation and Science

Field Value
Title The rhizobiome of herbaceous plants in Clovelly and Sterkspruit soils of the Stevenson–Hamilton supersite
Creator Vermeulen, Marcele Cason, Errol D. Swart, Wijnand J.
Subject Microbial ecology; Diversity Kruger National Park; Microbiome; Rhizobiome; 16S diversity; Stevenson–Hamilton Supersite; Granite catena
Description By attracting microorganisms from the surrounding soil via root exudates, the composition of microbial populations in the rhizosphere of plants is regulated and maintained according to the genotype of the plant and its abiotic soil environment. This project investigated the bacterial diversity of the rhizosphere microbiome (i.e. rhizobiome) of the three most common herbaceous plants (Kyphocarpa angustifolia [Amaranthaceae, Caryophyllales], Melhania acuminata [Malvaceae, Malvales] and Sida cordifolia [Malvacae, Malvales]) growing mutually in two different soil types (Clovelly [top] and Sterkspruit [bottom]) with differing abiotic characteristics at a granite catenal supersite in the Kruger National Park, South Africa. Two plant species (K. angustifolia and S. cordifolia) occurred at both the top and bottom sites, whilst M. acuminata only occurred at the top site. Ten rhizosphere samples were collected per plant from both the top and bottom sites, comprising a total of 50 samples. Biolog EcoPlates™ were used to assess differences in carbon source utilisation patterns by bacteria in the rhizobiome. For next-generation sequencing analysis, the DNA from four randomly selected rhizosphere soil samples from each plant species, at both the top and bottom sites, was combined to yield two samples from each locality for each species. Targeted metagenomic sequencing of the 16S rRNA gene region (V3 and V4 regions) was used to characterise the rhizobiome. Actinobacteria and Proteobacteria were the most dominant phyla in all rhizobiomes, and unique and shared operational taxonomic units were identified in all the rhizobiomes. Principal component analysis of the Biolog data revealed no disparity between the five rhizobiomes.Conservation implications: The results obtained in this study could play a role in micro-ecological scale conservation and management because microbial diversity in soils plays a vital role in shaping above-ground biodiversity and terrestrial ecosystem dynamics.
Publisher AOSIS Publishing
Contributor UFS Strategic Research Fund Mercia Coetzee (Central University of Technology, Bloemfontein, South Africa) Eddie Riddell and Navashni Govender (SANParks) Dr Jan-G Vermeulen (Microbial Biochemical and Food Biotechnology Department, University of the Free
Date 2020-10-29
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/koedoe.v62i2.1596
Source Koedoe; Vol 62, No 2 (2020); 9 pages 2071-0771 0075-6458
Language eng
Coverage Stevenson-Hamilton Supersite — —
Rights Copyright (c) 2020 Marcele Vermeulen, Errol D. Cason, Wijnand J. Swart