Use of Brucella abortus species specific polymerase chain reaction assay for the diagnosis of bovine brucellosis
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Use of Brucella abortus species specific polymerase chain reaction assay for the diagnosis of bovine brucellosis | |
| Creator | Chisi, Songelwayo L. Schmidt, Tracy Akol, George W. van Heerden, Henriette | |
| Description | Serology is primarily used in the diagnosis of bovine brucellosis. Bacterial culture and isolation is the gold standard in diagnosing brucellosis but, like serology, it does not offer complete (100%) diagnostic sensitivity and specificity. Polymerase chain reaction (PCR) has been suggested to offer better specificity and sensitivity. In this study, we evaluated the performance of Brucella abortus species specific (BaSS) PCR directly from different samples in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture. The BaSS PCR had a low diagnostic sensitivity (DSe) of 70%, but was able to identify vaccine strains using abomasal fluid from aborted foetuses and detect Brucella DNA from decomposing samples. The best sample for the BaSS PCR was abomasal fluid. | |
| Publisher | AOSIS | |
| Date | 2017-09-27 | |
| Identifier | 10.4102/jsava.v88i0.1433 | |
| Source | Journal of the South African Veterinary Association; Vol 88 (2017); 3 pages 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://jsava.co.za/index.php/jsava/article/view/1433/1995
https://jsava.co.za/index.php/jsava/article/view/1433/1994
https://jsava.co.za/index.php/jsava/article/view/1433/1996
https://jsava.co.za/index.php/jsava/article/view/1433/1989
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