The impact of four harvesting techniques on the cell viability and osteogenic behaviour of cells in autogenous bone grafts: A critical appraisal of an experimental study

Open Journal of Implant Dentistry


 
 
Field Value
 
Title The impact of four harvesting techniques on the cell viability and osteogenic behaviour of cells in autogenous bone grafts: A critical appraisal of an experimental study
 
Creator van Zyl, Andre W. Hartshorne, Johan Carrasco-Labra, Alonso
 
Subject — autogenous bone grafts; bone drilling; bone harvesting; bone milling; bone scraping
Description The investigators tested the null hypothesis that there would be no differences between the different bone harvesting techniques with regard to cell viability, cell activity and osteogenic potential of grafted cells. Bone grafts were harvested from the mandibles of 12 miniature pigs using four different harvesting techniques: bone milling, bone scraping, bone drilling (bone slurry) and piezosurgery. Cell viability was determined according to an immunoassay of released signalling molecules and gene expression that affect bone formation and resorption. The osteogenic activity of conditioned graft-sampled media was assessed in a bioassay using isolated bone cells. Cells in autogenous bone grafts obtained by using a bone mill and a bone scraper showed a higher viability and a stronger osteogenic potential than those from piezosurgery and bone drilling (slurry). This study contributed towards the understanding of the impact of harvesting techniques on the viability and osteogenic behaviour of grafted cells.
 
Publisher AOSIS
 
Contributor
Date 2013-03-26
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/octet-stream text/xml application/pdf
Identifier 10.4102/ojid.v1i1.6
 
Source Open Journal of Implant Dentistry; Vol 1, No 1 (2013); 3 pages 2226-4507 n/a
 
Language eng
 
Relation https://ojid.org/index.php/ojid/article/view/6/14 https://ojid.org/index.php/ojid/article/view/6/15 https://ojid.org/index.php/ojid/article/view/6/16 https://ojid.org/index.php/ojid/article/view/6/13
 
Coverage — — —
Rights Copyright (c) 2013 Andre W. van Zyl, Johan Hartshorne, Alonso Carrasco-Labra https://creativecommons.org/licenses/by/4.0